Код:

@echo off
setlocal enableextensions enabledelayedexpansion
set sSourceFile=E:\Песочница\0372\0001.txt
if exist "%sSourceFile%" (
for /f "usebackq tokens=3,4 delims=:" %%i in ("%sSourceFile%") do (
set sVar1=%%i
set sVar2=%%j
echo sVar1=[!sVar1!]
echo sVar2=[!sVar2!]
)
) else (
echo Source file [%sSourceFile%] not found.
exit /b 1
)
endlocal
exit /b 0